Effects of oxidative stress on sex-specific gene expression in the copepod Tigriopus californicus revealed by single individual RNA-seq.

ABSTRACT: Oxidative stress reflects the imbalance of pro-oxidants and antioxidants. Prolonged oxidative stress can induce cellular damage, diseases and aging, and the effects may be sex-specific. Tigriopus californicus has recently been proposed as an alternative model system for sex-specific studies due to the absence of sex chromosomes. In this study, we used comparative transcriptomic analyses to assess sex-specific transcriptional responses to oxidative stress. Male and female individuals were maintained separately in one of three treatments: 1) control conditions with an algae diet, 2) pro-oxidant (H₂O₂) conditions with an algae diet or 3) decreased antioxidant conditions (reduced carotenoids due to a yeast diet). Single individual RNA-seq was then conducted for twenty-fou

SUBMITTER: Li N 

PROVIDER: S-EPMC6697563 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature