Fragmentation Behavior and Gas-Phase Structures of Cationized Glycosphingolipids in Ozone-Induced Dissociation Mass Spectrometry.

ABSTRACT: The role of cationization in the fragmentation behavior of glycoconjugates is amply documented in collisional activation techniques but remains less explored in ozone-induced dissociation mass spectrometry (OzID-MS). OzID-MS has been used to elucidate the location of carbon-carbon double bonds in unsaturated lipids. Previously, we demonstrated the structural analysis of unsaturated glycosphingolipids using OzID-MS by mass-selecting the [M+Na]⁺ adduct for fragmentation. In this work, we aimed to examine the effect of different adducts, namely [M+Na]⁺, [M+Li]⁺, and [M+H]⁺ on the OzID-MS fragmentation behavior of a representative unsaturated glycosphingolipid, LacCer d18:1/18:1(9Z). Our data show that [M+H]⁺ primarily undergoes dehydratio

SUBMITTER: Barrientos RC 

PROVIDER: S-EPMC6697594 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature