Terbium chloride influences Clostridium difficile spore germination.

ABSTRACT: The germination of Clostridium difficile spores is an important stage of the C. difficile life cycle. In other endospore-forming bacteria, the composition of the medium in which the spores are generated influences the abundance of germination-specific proteins, thereby influencing the sensitivity of the spores towards germinants. In C. difficile media composition on the spores has only been reported to influence the number of spores produced. One of the measures of spore germination is the analysis of the release of DPA from the spore core. To detect DPA release in real time, terbium chloride is often added to the germination conditions because Tb³⁺ complexes with the released DPA and this can be detected using fluorescence measurements. Although C. difficile spores germinate in

SUBMITTER: Shrestha R 

PROVIDER: S-EPMC6697597 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature