A novel thermostable D-amino acid oxidase of the thermophilic fungus Rasamsonia emersonii strain YA.

ABSTRACT: D-Amino acid oxidase (DAAO) is a valuable flavoenzyme capable of being used in various practical applications, such as in determining D-amino acids and producing a material for semisynthetic cephalosporins, requiring higher thermal stability, higher catalytic activity, and broad substrate specificity. In this study, we isolated the thermophilic fungus Rasamsonia emersonii strain YA, which can grow on several D-amino acids as the sole nitrogen source, from a compost and characterized DAAO (ReDAAO) of the fungus. ReDAAO expressed in Escherichia coli exhibited significant oxidase activity against various neutral and basic D-amino acids, in particular hydrophobic D-amino acids. In addition, the enzyme also significantly acted on cephalosporin C, a starting material for semisynthetic antibiotic

SUBMITTER: Shimekake Y 

PROVIDER: S-EPMC6697736 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature