NADPH-dependent extracellular superoxide production is vital to photophysiology in the marine diatom Thalassiosira oceanica.

ABSTRACT: Reactive oxygen species (ROS) like superoxide drive rapid transformations of carbon and metals in aquatic systems and play dynamic roles in biological health, signaling, and defense across a diversity of cell types. In phytoplankton, however, the ecophysiological role(s) of extracellular superoxide production has remained elusive. Here, the mechanism and function of extracellular superoxide production by the marine diatom Thalassiosira oceanica are described. Extracellular superoxide production in T. oceanica exudates was coupled to the oxidation of NADPH. A putative NADPH-oxidizing flavoenzyme with predicted transmembrane domains and high sequence similarity to glutathione reductase (GR) was implicated in this process. GR was also linked to extracellular superoxide productio

SUBMITTER: Diaz JM 

PROVIDER: S-EPMC6697786 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature