Maintenance of translational elongation rate underlies the survival of Escherichia coli during oxidative stress.

ABSTRACT: To cope with harsh circumstances, bacterial cells must initiate cellular stress response programs, which demands the de novo synthesis of many stress defense proteins. Reactive oxygen species (ROS) is a universal environmental stressor for both prokaryotic cells and eukaryotic cells. However, the physiological burden that limits the survival of bacterial cells during oxidative stress remains elusive. Here we quantitatively characterize the cell growth and translational elongation rate of Escherichia coli cells treated with different doses of hydrogen peroxide. Cell growth is immediately arrested by low to moderate levels of hydrogen peroxide, but completely recovers after a certain lag time. The lag time depends positively on the dose of hydrogen peroxide. During the lag time, translationa

SUBMITTER: Zhu M 

PROVIDER: S-EPMC6698664 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature