Catalytic Knockdown of miR-21 by Artificial Ribonuclease: Biological Performance in Tumor Model.

ABSTRACT: Control of the expression of oncogenic small non-coding RNAs, notably microRNAs (miRNAs), is an attractive therapeutic approach. We report a design platform for catalytic knockdown of miRNA targets with artificial, sequence-specific ribonucleases. miRNases comprise a peptide [(LeuArg)₂Gly]₂ capable of RNA cleavage conjugated to the miRNA-targeted oligodeoxyribonucleotide, which becomes nuclease-resistant within the conjugate design, without resort to chemically modified nucleotides. Our data presented here showed for the first time a truly catalytic character of our miR-21-miRNase and its ability to cleave miR-21 in a multiple catalytic turnover mode. We demonstrate that miRNase targeted to miR-21 (miR-21-miRNase) knocked down malignant behavior of tumor cells, includ

SUBMITTER: Patutina OA 

PROVIDER: S-EPMC6698794 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature