Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies.

ABSTRACT: Sialoadhesin (Sn) is a surface receptor expressed on a subset of macrophages in steady state conditions. During inflammation and diseases, Sn is highly upregulated on macrophages and blood monocytes. Therefore, therapies using monoclonal antibodies (mAbs) to target Sn-positive (Sn+) cells are a potential strategy for targeted treatment. It has been shown that Sn internalizes after binding with a mAb, though it is not clear whether this is species-specific. In this study, new Sn-specific mAbs were developed and analyzed for cross-reactivity between species. In addition, the newly developed mAbs were compared to mAbs used in previous research for their epitope recognition and other Sn-specific characteristics. Both species-specific and cross-reactive antibodies could be identified. Furthermo

SUBMITTER: De Schryver M 

PROVIDER: S-EPMC6698821 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature