Epitope Sequences in Dengue Virus NS1 Protein Identified by Monoclonal Antibodies.

ABSTRACT: Dengue nonstructural protein 1 (NS1) is a multi-functional glycoprotein with essential functions both in viral replication and modulation of host innate immune responses. NS1 has been established as a good surrogate marker for infection. In the present study, we generated four anti-NS1 monoclonal antibodies against recombinant NS1 protein from dengue virus serotype 2 (DENV2), which were used to map three NS1 epitopes. The sequence ¹⁹³AVHADMGYWIESALNDT²⁰⁹ was recognized by monoclonal antibodies 2H5 and 4H1BC, which also cross-reacted with Zika virus (ZIKV) protein. On the other hand, the sequence ²⁵VHTWTEQYKFQPES³⁸ was recognized by mAb 4F6 that did not cross react with ZIKV. Lastly, a previously unidentified DENV2 NS1-specific epitope, represente

SUBMITTER: Rocha LB 

PROVIDER: S-EPMC6698852 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature