Controlling the Glycosylation Profile in mAbs Using Time-Dependent Media Supplementation.

ABSTRACT: In order to meet desired drug product quality targets, the glycosylation profile of biotherapeutics such as monoclonal antibodies (mAbs) must be maintained consistently during manufacturing. Achieving consistent glycan distribution profiles requires identifying factors that influence glycosylation, and manipulating them appropriately via well-designed control strategies. Now, the cell culture media supplement, MnCl2, is known to alter the glycosylation profile in mAbs generally, but its effect, particularly when introduced at different stages during cell growth, has yet to be investigated and quantified. In this study, we evaluate the effect of time-dependent addition of MnCl2 on the glycan profile quantitatively, using factorial design experiments. Our results show that MnCl2 addition dur

SUBMITTER: Radhakrishnan D 

PROVIDER: S-EPMC6698858 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature