Distinctive functional deficiencies in axonal conduction associated with two forms of cerebral white matter injury.

ABSTRACT:

Aims

This study determines whether assessment with compound action potentials (CAPs) can distinguish two different forms of cerebral white matter injury at the functional levels.

Methods

A pure demyelination model was induced in C57/BL6 adult mice by dietary supplementation of cuprizone (0.2%) for 6 weeks. Callosal L-N5-(1-Iminoethyl) ornithine (L-NIO) hydrochloride (27 mg/mL) was injected into the corpus callosum (CC) to induce a focal white matter stroke (WMS), resulting in both demyelination and axonal injury. White matter integrity was assessed by performing CAP recording, electron microscopy, and immunohistological and luxol fast blue (LFB) staining.

Results

Immunohistological and electron microscopic analyses confirmed the induction of robust demyelination in CC

SUBMITTER: Mu HF 

PROVIDER: S-EPMC6698976 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature