Genetic variants of the oppA gene are involved in metabolic regulation of surfactin in Bacillus subtilis.

ABSTRACT:

Background

Bacillus subtilis 916 has been identified as an effective biocontrol agent against Rhizoctonia solani, the causal pathogen of rice sheath blight, under greenhouse and field conditions. HPLC analysis showed that surfactin, a member of the lipopeptide family produced by B. subtilis, was the major antimicrobial substance.

Results

Previously, we obtained a mutant strain of B. subtilis 916, Bs-H74, which produced significantly more surfactin than the wild type and presented 10% stronger inhibitory activity against R. solani. To explore the molecular mechanism underlying the higher surfactin productivity in the mutant, high-throughput proteomic analysis was carried out to analyze the differential protein expression. Our results showed that several differentially express

SUBMITTER: Wang X 

PROVIDER: S-EPMC6699124 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature