Phosphatidylinositol 4,5-bisphosphate (PIP₂) and Ca²⁺ are both required to open the Cl^- channel TMEM16A.

ABSTRACT: Transmembrane member 16A (TMEM16A) is a widely expressed Ca²⁺-activated Cl^- channel with various physiological functions ranging from mucosal secretion to regulating smooth muscle contraction. Understanding how TMEM16A controls these physiological processes and how its dysregulation may cause disease requires a detailed understanding of how cellular processes and second messengers alter TMEM16A channel gating. Here we assessed the regulation of TMEM16A gating by recording Ca²⁺-evoked Cl^- currents conducted by endogenous TMEM16A channels expressed in Xenopus laevis oocytes, using the inside-out configuration of the patch clamp technique. During continuous application of Ca²⁺, we found that TMEM16A-conducted currents decay shortly

SUBMITTER: Tembo M 

PROVIDER: S-EPMC6699839 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature