The mechanosensitive channel YbdG from Escherichia coli has a role in adaptation to osmotic up-shock.

ABSTRACT: Mechanosensitive channels play an important role in the adaptation of cells to hypo-osmotic shock. Among members of this channel family in Escherichia coli, the exact function and physiological role of the mechanosensitive channel homolog YbdG remain unclear. Characterization of YbdG's physiological role has been hampered by its lack of measurable transport activity. Using a nitrosoguanidine mutagenesis-aided screen in combination with next-generation sequencing, here we isolated a mutant with a point mutation in ybdG This mutation (resulting in a I167T change) conferred sensitivity to high osmotic stress, and the mutant cells differed from WT cells in morphology during hyperosmotic stress at alkaline pH. Interestingly, unlike the cells containing the I167T variant, a null-

SUBMITTER: Amemiya S 

PROVIDER: S-EPMC6699842 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature