Zinc-dependent activation of the Pho8 alkaline phosphatase in Schizosaccharomyces pombe.

ABSTRACT: Genome-wide analyses have revealed that during metal ion starvation, many cells undergo programmed changes in their transcriptome or proteome that lower the levels of abundant metalloproteins, conserving metal ions for more critical functions. Here we investigated how changes in cellular zinc status affect the expression and activity of the zinc-requiring Pho8 alkaline phosphatase from fission yeast (Schizosaccharomyces pombe). In S. pombe, Pho8 is a membrane-tethered and processed glycoprotein that resides in the vacuole. Using alkaline phosphatase activity assays along with various biochemical analyses, we found that Pho8 is active when zinc is plentiful and inactive when zinc is limited. Although Pho8 activity depended on zinc, we also found that higher levels of pho8</

SUBMITTER: Hu YM 

PROVIDER: S-EPMC6699849 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature