A flow through device for simultaneous dielectrophoretic cell trapping and AC electroporation.

ABSTRACT: Isolation of cells and their transfection in a controlled manner is an integral step in cell biotechnology. Electric field approaches such as dielectrophoresis (DEP) offers a more viable method for targeted immobilization of cells without any labels. For transfection of cells to incorporate exogenous materials, electrical methods such as electroporation, are preferred over chemical and viral delivery methods since they minimally affect cell viability and can target many types. However prior approaches to both methods required multiple excitation sources, an AC source for DEP-based trapping and another DC source for electroporation. In this paper, we present a first of its kind flow through lab-on-chip platform using a single AC excitation source for combined trapping using negative dielect

SUBMITTER: Punjiya M 

PROVIDER: S-EPMC6700080 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature