Evaluation of antigenic differences between wild and Sabin vaccine strains of poliovirus using the pseudovirus neutralization test.

ABSTRACT: In the endgame of global polio eradication, serosurveillance is essential to monitor each country's vulnerability to poliomyelitis outbreaks. Previously, we developed pseudovirus poliovirus (PV) neutralization test (pPNT) with type 1, 2, and 3 PV pseudovirus (PV_pv), which possess a luciferase-encoding PV replicon in the capsids of wild-type strains (PV_pv[WT]), showing that pPNT with type 2 and 3 PV_pv(WT) but not type 1 shows high correlation with the conventional PV neutralization test (cPNT) performed with vaccine strains. Here, we analyse the antigenicity of PV_pv(WT) and PV_pv with capsid proteins of Sabin vaccine strains (PV_pv[Sabin]) in human serum. Type 2 and 3 PV_pv(WT) and PV_pv(Sabin) show similar

SUBMITTER: Arita M 

PROVIDER: S-EPMC6700111 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature