A Multi-center Study on the Reproducibility of Drug-Response Assays in Mammalian Cell Lines.

ABSTRACT: Evidence that some high-impact biomedical results cannot be repeated has stimulated interest in practices that generate findable, accessible, interoperable, and reusable (FAIR) data. Multiple papers have identified specific examples of irreproducibility, but practical ways to make data more reproducible have not been widely studied. Here, five research centers in the NIH LINCS Program Consortium investigate the reproducibility of a prototypical perturbational assay: quantifying the responsiveness of cultured cells to anti-cancer drugs. Such assays are important for drug development, studying cellular networks, and patient stratification. While many experimental and computational factors impact intra- and inter-center reproducibility, the factors most difficult to identify and control are t

SUBMITTER: Niepel M 

PROVIDER: S-EPMC6700527 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature