A model of pulldown alignments from SssI-treated DNA improves DNA methylation prediction.

ABSTRACT:

Background

Protein pulldown using Methyl-CpG binding domain (MBD) proteins followed by high-throughput sequencing is a common method to determine DNA methylation. Algorithms have been developed to estimate absolute methylation level from read coverage generated by affinity enrichment-based techniques, but the most accurate one for MBD-seq data requires additional data from an SssI-treated Control experiment.

Results

Using our previous characterizations of Methyl-CpG/MBD2 binding in the context of an MBD pulldown experiment, we build a model of expected MBD pulldown reads as drawn from SssI-treated DNA. We use the program BayMeth to evaluate the effectiveness of this model by substituting calculated SssI Control data for the observed SssI Control data. By comparing methylatio

SUBMITTER: Moreland BS 

PROVIDER: S-EPMC6700779 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature