bla_NDM-5 carried by a hypervirulent Klebsiella pneumoniae with sequence type 29.

ABSTRACT:

Background

A carbapenem-resistant hypermucoviscous Klebsiella pneumoniae isolate was recovered from human sputum.

Methods

Whole genome sequencing of this isolate was carried out to reveal its clonal background, antimicrobial resistance determinants and virulence factors. Virulence assays were performed using wax moth larvae. The transfer of bla_NDM-5 between bacterial strains was tested using conjugation. 59 genome assemblies of ST29 K. pneumoniae and 230 IncX3 plasmids regardless of the carriage of resistance gene were employed for phylogenetic analysis, respectively.

Results

The strain carried a virulence plasmid pVir-SCNJ1 bearing the virulence gene rmpA and exhibited a high virulence in wax moth. This hypervirulent strain belo

SUBMITTER: Yuan Y 

PROVIDER: S-EPMC6701021 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature