Engineering Geobacillus thermoglucosidasius for direct utilisation of holocellulose from wheat straw.

ABSTRACT:

Background

A consolidated bioprocessing (CBP), where lignocellulose is converted into the desired product(s) in a single fermentative step without the addition of expensive degradative enzymes, represents the ideal solution of renewable routes to chemicals and fuels. Members of the genus Geobacillus are able to grow at elevated temperatures and are able to utilise a wide range of oligosaccharides derived from lignocellulose. This makes them ideally suited to the development of CBP.

Results

In this study, we engineered Geobacillus thermoglucosidasius NCIMB 11955 to utilise lignocellulosic biomass, in the form of nitric acid/ammonia treated wheat straw to which expensive hydrolytic enzymes had not been added. Two different strains, BZ9 and BZ10, were generated by

SUBMITTER: Bashir Z 

PROVIDER: S-EPMC6701081 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature