GC-MS method for metabolic profiling of mouse femoral head articular cartilage reveals distinct effects of tissue culture and development.

ABSTRACT:

Objective

The metabolic profile of cartilage is important to define as it relates to both normal and pathophysiological conditions. Our aim was to develop a precise, high-throughput method for gas/chromatography-mass/spectrometry (GC-MS) semi-targeted metabolic profiling of mouse cartilage.

Method

Femoral head (hip) cartilage was isolated from 5- and 15-week-old male C57BL/6J mice immediately after death for in vivo analyses. In vitro conditions were evaluated in 5-week-old samples cultured ±10% fetal bovine serum (FBS). We optimized cartilage processing for GC-MS analysis and evaluated group-specific differences by multivariate and parametric statistical analyses.

Results

55 metabolites were identified in pooled cartilage (4 animals per sample), with 29 metabolites s

SUBMITTER: Batushansky A 

PROVIDER: S-EPMC6702098 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature