Productive HBV infection of well-differentiated, hNTCP-expressing human hepatoma-derived (Huh7) cells.

ABSTRACT: Feasible and effective cell models for hepatitis B virus (HBV) infection are required for investigating the complete lifecycle of this virus, including the early steps of viral entry. Resistance to dimethyl sulfoxide/polyethylene glycol (DMSO/PEG), hNTCP expression, and a differentiated state are the limiting factors for successful HBV infection models. In the present study, we used a hepatoma cell line (Huh7D^hNTCP) to overcome these limiting factors so that it exhibits excellent susceptibility to HBV infection. To achieve this goal, different hepatoma cell lines were tested with 2.5% DMSO / 4% PEG8000, and one resistant cell line (Huh7D) was used to construct a stable hNTCP-expressing cell line (Huh7D^hNTCP) using a recombinant lentivirus system. Then, the morphologic

SUBMITTER: Zhou M 

PROVIDER: S-EPMC6702241 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature