A Rapid Method for Label-Free Enrichment of Rare Trophoblast Cells from Cervical Samples.

ABSTRACT: Extravillous trophoblasts (EVTs) have the potential to provide the entire fetal genome for prenatal testing. Previous studies have demonstrated the presence of EVTs in the cervical canal and the ability to retrieve a small quantity of these cells by cervical sampling. However, these small quantities of trophoblasts are far outnumbered by the population of cervical cells in the sample, making isolation of the trophoblasts challenging. We have developed a method to enrich trophoblast cells from a cervical sample using differential settling of the cells in polystyrene wells. We tested the addition of small quantities of JEG-3 trophoblast cell line cells into clinical samples from standard Pap tests taken at 5 to 20 weeks of gestation to determine the optimal work flow. We observed that a 4 mi

SUBMITTER: Bailey-Hytholt CM 

PROVIDER: S-EPMC6702343 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature