Determination of iohexol by capillary blood microsampling and UHPLC-MS/MS.

ABSTRACT: One of the most important tools used to evaluate kidney function in the context of chronic kidney disease or other renal function related pathologies is the exploration of glomerular filtration rate (GFR). Iohexol is up to this moment a good candidate molecule for the GFR assessment since it exhibits minimum protein binding rates and minimum extra-renal clearance, being neither secreted nor reabsorbed at the tubular level. This study proposes and evaluates a new LC-MS/MS method for the iohexol determination from capillary blood, prelevated using volumetric absorbative microsampling (VAMS) systems. As an alternative to VAMS, a brand new HemaPEN^® device for micro-prelevation was also tested. A new high throughput sample preparation protocol adapted for iohexol quantification from

SUBMITTER: Ion V 

PROVIDER: S-EPMC6702420 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature