Top-Down Characterization of Lipooligosaccharides from Antibiotic-Resistant Bacteria.

ABSTRACT: Modification of structures of lipooligosaccharides (LOS) represents one prevalent mechanism by which Gram-negative bacteria can become resistant to key antibiotics. Owing to the significant complexity of LOS, the structural characterization of these amphipathic lipids has largely focused on elucidation of the lipid A substructures. Analysis of intact LOS enables detection of core oligosaccharide modifications and gives insight into the heterogeneity that results from combinations of lipid A and oligosaccharide substructures. Top-down analysis of intact LOS also provides the opportunity to determine unknown oligosaccharide structures, which is particularly advantageous in the context of glycoconjugate vaccine development. Advances in mass spectrometry technologies, including the development

SUBMITTER: Klein DR 

PROVIDER: S-EPMC6702669 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature