Cyclic nucleotide-gated ion channel 6 mediates thermotolerance in Arabidopsis seedlings by regulating nitric oxide production via cytosolic calcium ions.

ABSTRACT:

Background

We previously reported the involvement of nitric oxide (NO) and cyclic nucleotide-gated ion channel 6 (CNGC6) in the responses of plants to heat shock (HS) exposure. To elucidate their relationship with heat tolerance in Arabidopsis thaliana, we examined the effects of HS on several groups of seedlings: wild type, cngc6, and cngc6 complementation and overexpression lines.

Results

After HS exposure, the level of NO was lower in cngc6 seedlings than in wild-type seedlings but significantly elevated in the transgenic lines depending on CNGC6 expression level. The treatment of seeds with calcium ions (Ca²⁺) enhanced the NO level in Arabidopsis seedlings under HS conditions, whereas treatment with EGTA (a Ca²⁺ chelator) reduced it, implicating tha

SUBMITTER: Peng X 

PROVIDER: S-EPMC6702746 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature