Cellular Dynamics of Mouse Trophoblast Stem Cells: Identification of a Persistent Stem Cell Type.

ABSTRACT: Mouse trophoblast stem cells (TSCs) proliferate indefinitely in vitro, despite their highly heterogeneous nature. In this study, we sought to characterize TSC colony types by using methods based on cell biology and biochemistry for a better understanding of how TSCs are maintained over multiple passages. Colonies of TSCs could be classified into four major types: type 1 is compact and dome-shaped, type 4 is flattened but with a large multilayered cell cluster, and types 2 and 3 are their intermediates. A time-lapse analysis indicated that type 1 colonies predominantly appeared after passaging, and a single type 1 colony gave rise to all other types. These colony transitions were irreversible, but at least some type 1 colonies persisted throughout culture. The typical cells comprising type

SUBMITTER: Motomura K 

PROVIDER: S-EPMC6702784 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature