Molecular identification, characterization, and structure analysis of house musk shrew (Suncus murinus) leptin.

ABSTRACT:

Objective

House musk shrew (Suncus murinus), a small experimental animal with low body fat, may be a possible model for human lipodystrophy. Leptin is an adipocyte-derived hormone thought to have an important role in the pathophysiology of lipodystrophy. The objectives of this study were to clarify the structure and distribution of suncus leptin.

Materials and methods

To determine the primary structure of suncus leptin, we cloned the suncus Lep cDNA using the rapid amplification of cDNA ends method. The obtained amino acid (aa) sequence was compared with other mammals and the protein structure prediction was performed.

Results

The suncus Lep cDNA encodes 170 aa. The putative suncus leptin precursor has a predicted signal peptide of 21 aa, and the

SUBMITTER: Saga S 

PROVIDER: S-EPMC6702923 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature