Lack of Renal Tubular Glucocorticoid Receptor Decreases the Thiazide-Sensitive Na⁺/Cl^- Cotransporter NCC and Transiently Affects Sodium Handling.

ABSTRACT: Chronic glucocorticoid infusion impairs NCC activity and induces a non-dipping profile in mice, suggesting that glucocorticoids are essential for daily blood pressure variations. In this paper, we studied mice lacking the renal tubular glucocorticoid receptor (GR) in adulthood (GR knockouts, Nr3c1 ^Pax8/LC1 ). Upon standard salt diet, Nr3c1 ^Pax8/LC1 mice grow normally, but show reduced NCC activity despite normal plasma aldosterone levels. Following diet switch to low sodium, Nr3c1 ^Pax8/LC1 mice exhibit a transient but significant reduction in the activity of NCC and expression of NHE3 and NKCC2 accompanied by significant increased Spak activity. This is followed by transiently increased urinary sodium excretion and

SUBMITTER: Canonica J 

PROVIDER: S-EPMC6702950 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature