Crosslinked Chitosan-PEG Hydrogel for Culture of Human Glioblastoma Cell Spheroids and Drug Screening.

ABSTRACT: Two-dimensional monolayer cell cultures are routinely utilized for preclinical cancer drug screening, but the results often do not translate well when drugs are tested in vivo. To address this limitation, a biocompatible chitosan-PEG hydrogel (CSPG gel) was synthesized to create a gel that can be easily dispensed into 96-well plates at room temperature and neutral pH. The stiffness of this gel was tailored to be within the stiffness range of human glioblastoma tissue to promote the formation of tumor spheroids. Differences in cell morphology, proliferation rate, and dose-dependent drug cytotoxicity were compared among cell spheroids grown on CSPG gels, cells in monolayer culture on tissue culture polystyrene and cells cultured on Matrigel. Tumor spheroids on CSPG gels displayed statistical

SUBMITTER: Chang FC 

PROVIDER: S-EPMC6703847 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature