Targeted Deletion of Hepatocyte Abca1 Increases Plasma HDL (High-Density Lipoprotein) Reverse Cholesterol Transport via the LDL (Low-Density Lipoprotein) Receptor.

ABSTRACT:

Objective

The role of hepatocyte Abca1 (ATP binding cassette transporter A1) in trafficking hepatic free cholesterol (FC) into plasma versus bile for reverse cholesterol transport (RCT) is poorly understood. We hypothesized that hepatocyte Abca1 recycles plasma HDL-C (high-density lipoprotein cholesterol) taken up by the liver back into plasma, maintaining the plasma HDL-C pool, and decreasing HDL-mediated RCT into feces. Approach and Results: Chow-fed hepatocyte-specific Abca1 knockout (HSKO) and control mice were injected with human HDL radiolabeled with ¹²⁵I-tyramine cellobiose (¹²⁵I-TC; protein) and ³H-cholesteryl oleate (³H-CO). ¹²⁵I-TC and ³H-CO plasma decay, plasma HDL ³H-CO selective clearance (ie,

SUBMITTER: Bashore AC 

PROVIDER: S-EPMC6703909 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature