Functional analysis of isoprenoid precursors biosynthesis by quantitative metabolomics and isotopologue profiling.

ABSTRACT:

Introduction

Isoprenoids are amongst the most abundant and diverse biological molecules and are involved in a broad range of biological functions. Functional understanding of their biosynthesis is thus key in many fundamental and applicative fields, including systems biology, medicine and biotechnology. However, available methods do not yet allow accurate quantification and tracing of stable isotopes incorporation for all the isoprenoids precursors.

Objectives

We developed and validated a complete methodology for quantitative metabolomics and isotopologue profiling of isoprenoid precursors in the yeast Saccharomyces cerevisiae.

Methods

This workflow covers all the experimental and computational steps from sample collection and preparation to data acquisition and proce

SUBMITTER: Castano-Cerezo S 

PROVIDER: S-EPMC6704079 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature