High-speed device synchronization in optical microscopy with an open-source hardware control platform.

ABSTRACT: Azimuthal beam scanning eliminates the uneven excitation field arising from laser interference in through-objective total internal reflection fluorescence (TIRF) microscopy. The same principle can be applied to scanning angle interference microscopy (SAIM), where precision control of the scanned laser beam presents unique technical challenges for the builders of custom azimuthal scanning microscopes. Accurate synchronization between the instrument computer, beam scanning system and excitation source is required to collect high quality data and minimize sample damage in SAIM acquisitions. Drawing inspiration from open-source prototyping systems, like the Arduino microcontroller boards, we developed a new instrument control platform to be affordable, easily programmed, and broadly useful, bu

SUBMITTER: Colville MJ 

PROVIDER: S-EPMC6704125 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature