Binding of the protein ICln to α-integrin contributes to the activation of ICl_swell current.

ABSTRACT: ICl_swell is the chloride current induced by cell swelling, and plays a fundamental role in several biological processes, including the regulatory volume decrease (RVD). ICln is a highly conserved, ubiquitously expressed and multifunctional protein involved in the activation of ICl_swell. In platelets, ICln binds to the intracellular domain of the integrin αIIb chain, however, whether the ICln/integrin interaction plays a role in RVD is not known. Here we show that a direct molecular interaction between ICln and the integrin α-chain is not restricted to platelets and involves highly conserved amino acid motifs. Integrin α recruits ICln to the plasma membrane, thereby facilitating the activation of ICl_swell during hypotonicity. Perturbation of the ICln/integri

SUBMITTER: Schedlbauer A 

PROVIDER: S-EPMC6704128 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature