A non-linear mathematical model using optical sensor to predict heart decellularization efficacy.

ABSTRACT: One of the main problems of the decellularization technique is the subjectivity of the final evaluation of its efficacy in individual organs. This problem can result in restricted cell repopulation reproducibility and worse responses to transplant tissues. Our proposal is to analyze the optical profiles produced by hearts during perfusion decellularization, as an additional method for evaluating the decellularization process of each individual organ. An apparatus comprised of a structured LED source and photo detector on an adjustable base was developed to capture the relationship between transmitted light during the perfusion of murine hearts, and residual DNA content. Voltage-time graphic records were used to identify a nonlinear mathematical model to discriminate between decellularizati

SUBMITTER: Pereira RHA 

PROVIDER: S-EPMC6704168 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature