Precise co-registration of mass spectrometry imaging, histology, and laser microdissection-based omics.

ABSTRACT: Mass spectrometry imaging (MSI) is an analytical technique for the unlabeled and multiplex imaging of molecules in biological tissue sections. It therefore enables the spatial and molecular annotations of tissues complementary to histology. It has already been shown that MSI can guide subsequent material isolation technologies such as laser microdissection (LMD) to enable a more in-depth molecular characterization of MSI-highlighted tissue regions. However, with MSI now reaching spatial resolutions at the single-cell scale, there is a need for a precise co-registration between MSI and the LMD. As proof-of-principle, MSI of lipids was performed on a breast cancer tissue followed by a segmentation of the data to detect molecularly distinct segments within its tumor areas. After image process

SUBMITTER: Dewez F 

PROVIDER: S-EPMC6704276 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature