Optimization of enzymatic hydrolysis of cellulosic fraction obtained from stranded driftwood feedstocks for lipid production by Solicoccozyma terricola.

ABSTRACT: Stranded driftwood feedstocks may represent, after pretreatment with steam explosion and enzymatic hydrolysis, a cheap C-source for producing biochemicals and biofuels using oleaginous yeasts. The hydrolysis was optimized using a response surface methodology (RSM). The solid loading (SL) and the dosage of enzyme cocktail (ED) were variated following a central composite design (CCD) aimed at optimizing the conversion of carbohydrates into lipids (Y_L) by the yeast Solicoccozyma terricola DBVPG 5870. A second-order polynomial equation was computed for describing the effect of ED and SL on Y_L. The best combination (ED = 3.10%; SL = 22.07%) for releasing the optimal concentration of carbohydrates which gave the highest predicted Y_L (27.32%) was then valid

SUBMITTER: Tasselli G 

PROVIDER: S-EPMC6704348 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature