In-situ 2D bacterial crystal growth as a function of protein concentration: An atomic force microscopy study.

ABSTRACT: The interplay between protein concentration and (observation) time has been investigated for the adsorption and crystal growth of the bacterial SbpA proteins on hydrophobic fluoride-functionalized SiO₂ surfaces. For this purpose, atomic force microscopy (AFM) has been performed in real-time for monitoring protein crystal growth at different protein concentrations. Results reveal that (1) crystal formation occurs at concentrations above 0.08 µM and (2) the compliance of the formed crystal decreases by increasing protein concentration. All the crystal domains observed presented similar lattice parameters (being the mean value for the unit cell: a = 14.8 ± 0.5 nm, b = 14.7 ± 0.5 nm, γ = 90 ° ± 2). Protein film formation is shown to take place from initial nucleation points which or

SUBMITTER: Moreno-Cencerrado A 

PROVIDER: S-EPMC6704365 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature