Clustering of IRE1α depends on sensing ER stress but not on its RNase activity.

ABSTRACT: The sensors of the unfolded protein response react to endoplasmic reticulum (ER) stress by transient activation of their enzymatic activities, which initiate various signaling cascades. In addition, the sensor IRE1α exhibits stress-induced clustering in a transient time frame similar to activation of its endoRNase activity. Previous work had suggested that the clustering response and RNase activity of IRE1α are functionally linked, but here we show that they are independent of each other and have different behaviors and modes of activation. Although both clustering and the RNase activity are responsive to luminal stress conditions and to depletion of the ER chaperone binding protein, RNase-inactive IRE1α still clusters and, conversely, full RNase activity can be accomplished without cluste

SUBMITTER: Ricci D 

PROVIDER: S-EPMC6704461 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature