A novel RNA variant of human concentrative nucleoside transporter 1 (hCNT1) that is a potential cancer biomarker.

ABSTRACT:

Background

The human concentrative nucleoside transporter 1 (hCNT1) a product of the SLC28A1 gene is one of the three concentrative nucleoside transporters, with a substrate specificity for physiological pyrimidine nucleosides. It has recently been implicated in tumor suppression. We have unraveled a splice variant RNA transcript that is overexpressed in some tumor tissues and some cancer cells. This study established  that observation.

Methods

We examined several clones of hCNT1 generated from RT-PCR of total RNA from human kidney tissue purchased from Ambion. The resulting cDNA clones were then sequenced, and a variant that retained intron 4, and skipped some exons fully or partly, specifically exons 5 and 13 were completely missed and only part of exon 6 was splice

SUBMITTER: Wang C 

PROVIDER: S-EPMC6704654 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature