Reduction of metastatic potential by inhibiting EGFR/Akt/p38/ERK signaling pathway and epithelial-mesenchymal transition after carbon ion exposure is potentiated by PARP-1 inhibition in non-small-cell lung cancer.

ABSTRACT:

Background

Carbon ion (¹²C) radiotherapy is becoming very promising to kill highly metastatic cancer cells keeping adjacent normal cells least affected. Our previous study shows that combined PARP-1 inhibition with ¹²C ion reduces MMP-2,-9 synergistically in HeLa cells but detailed mechanism are not clear. To understand this mechanism and the rationale of using PARP-1 inhibitor with ¹²C ion radiotherapy for better outcome in controlling metastasis, we investigated metastatic potential in two non-small cell lung cancer (NSCLC) A549 and H1299 (p53-deficient) cells exposed with ¹²C ion in presence and absence of PARP-1 inhibition using siRNA or olaparib.

Methods

We monitored cell proliferation, in-vitro cell migration, wound healing,

SUBMITTER: Chowdhury P 

PROVIDER: S-EPMC6704719 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature