Isolation, Culture and Functional Characterization of Glia and Endothelial Cells From Adult Pig Brain.

ABSTRACT: Primary cultures of glial and endothelial cells are important tools for basic and translational neuroscience research. Primary cell cultures are usually generated from rodent brain although considerable differences exist between human and rodent glia and endothelial cells. Because many translational research projects aim to identify mechanisms that eventually lead to diagnostic and therapeutic approaches to target human diseases, glia, and endothelial cultures are needed that better reflect the human central nervous system (CNS). Pig brain is easily accessible and, in many aspects, close to the human brain. We established an easy and cost-effective method to isolate and culture different primary glial and endothelial cells from adult pig brain. Oligodendrocyte, microglia, astrocyte, and en

SUBMITTER: Tanti GK 

PROVIDER: S-EPMC6705213 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature