Neural Deletion of Glucose Transporter Isoform 3 Creates Distinct Postnatal and Adult Neurobehavioral Phenotypes.

ABSTRACT: We created a neural-specific conditional murine glut3 (Slc2A3) deletion (glut3 ^{flox/flox/nestin-Cre+}) to examine the effect of a lack of Glut3 on neurodevelopment. Compared with age-matched glut3 ^flox/flox = WT and heterozygotes (glut3 ^{flox/+/nestin-Cre+}), we found that a >90% reduction in male and female brain Glut3 occurred by postnatal day 15 (PN15) in glut3 ^{flox/flox/nestin-Cre+} This genetic manipulation caused a diminution in brain weight and cortical thickness at PN15, a reduced number of dendritic spines, and fewer ultrasonic vocalizations. Patch-clamp recordings of cortical pyramidal neurons revealed increased frequency of bicuculline-induced paroxysmal discharges as well as reduced latency, attes

SUBMITTER: Shin BC 

PROVIDER: S-EPMC6706001 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature