Culturing Simpler and Bacterial Wilt Suppressive Microbial Communities from Tomato Rhizosphere.

ABSTRACT: Plant phenotype is affected by a community of associated microorganisms which requires dissection of the functional fraction. In this study, we aimed to culture the functionally active fraction of an upland soil microbiome, which can suppress tomato bacterial wilt. The microbiome fraction (MF) from the rhizosphere of Hawaii 7996 treated with an upland soil or forest soil MF was successively cultured in a designed modified M9 (MM9) medium partially mimicking the nutrient composition of tomato root exudates. Bacterial cells were harvested to amplify V3 and V4 regions of 16S rRNA gene for QIIME based sequence analysis and were also treated to Hawaii 7996 prior to Ralstonia solanacearum inoculation. The disease progress indicated that the upland MM9 1^st transfer suppressed th

SUBMITTER: Roy N 

PROVIDER: S-EPMC6706014 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature