Development and validation of an allele-specific PCR assay for genotyping a promoter and exonic single nucleotide polymorphisms of MGMT gene.

ABSTRACT: DNA repair protein O ⁶-methylguanine-DNA methyltransferase (MGMT) specifically remove the methyl/alkyl group from the O ⁶-position of guanine and restore the guanine to its normal form without causing DNA strand breaks. Relationship between MGMT activity and resistance to alkylating therapeutic agents is well established. Non-availability of simple, cost-effective and efficient methods of genotyping may hinder investigations on genotype-phenotype associations. No simple genotyping procedures such as allele-discrimination Taqman Assays were available for two genetic variations in MGMT gene that had previously demonstrated to be affecting its function and expression. These two variants were included to genotype in a clinical study (Clinicaltrail.gov ID: NC

SUBMITTER: Tyagi AK 

PROVIDER: S-EPMC6706101 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature