Spatiotemporal control of mitotic exit during anaphase by an aurora B-Cdk1 crosstalk.

ABSTRACT: According to the prevailing 'clock' model, chromosome decondensation and nuclear envelope reformation when cells exit mitosis are byproducts of Cdk1 inactivation at the metaphase-anaphase transition, controlled by the spindle assembly checkpoint. However, mitotic exit was recently shown to be a function of chromosome separation during anaphase, assisted by a midzone Aurora B phosphorylation gradient - the 'ruler' model. Here we found that Cdk1 remains active during anaphase due to ongoing APC/C^Cdc20- and APC/C^Cdh1-mediated degradation of B-type Cyclins in Drosophila and human cells. Failure to degrade B-type Cyclins during anaphase prevented mitotic exit in a Cdk1-dependent manner. Cyclin B1-Cdk1 localized at the spindle midzone in an Aurora B-dependent manner,

SUBMITTER: Afonso O 

PROVIDER: S-EPMC6706241 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature