Pol II phosphorylation regulates a switch between transcriptional and splicing condensates.

ABSTRACT: The synthesis of pre-mRNA by RNA polymerase II (Pol II) involves the formation of a transcription initiation complex, and a transition to an elongation complex^1-4. The large subunit of Pol II contains an intrinsically disordered C-terminal domain that is phosphorylated by cyclin-dependent kinases during the transition from initiation to elongation, thus influencing the interaction of the C-terminal domain with different components of the initiation or the RNA-splicing apparatus^5,6. Recent observations suggest that this model provides only a partial picture of the effects of phosphorylation of the C-terminal domain^7-12. Both the transcription-initiation machinery and the splicing machinery can form phase-separated condensates that contain large numbers of co

SUBMITTER: Guo YE 

PROVIDER: S-EPMC6706314 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature