Virus-like particle size and molecular weight/mass determination applying gas-phase electrophoresis (native nES GEMMA).

ABSTRACT: (Bio-)nanoparticle analysis employing a nano-electrospray gas-phase electrophoretic mobility molecular analyzer (native nES GEMMA) also known as nES differential mobility analyzer (nES DMA) is based on surface-dry analyte separation at ambient pressure. Based on electrophoretic principles, single-charged nanoparticles are separated according to their electrophoretic mobility diameter (EMD) corresponding to the particle size for spherical analytes. Subsequently, it is possible to correlate the (bio-)nanoparticle EMDs to their molecular weight (M_W) yielding a corresponding fitted curve for an investigated analyte class. Based on such a correlation, (bio-)nanoparticle M_W determination via its EMD within one analyte class is possible. Turning our attention to icosahedral,

SUBMITTER: Weiss VU 

PROVIDER: S-EPMC6706367 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature