Real-time imaging of photosynthetic oxygen evolution from spinach using LSI-based biosensor.

ABSTRACT: The light-driven splitting of water to oxygen (O₂) is catalyzed by a protein-bound tetra-manganese penta-oxygen calcium (Mn₄O₅Ca) cluster in Photosystem II. In the current study, we used a large-scale integration (LSI)-based amperometric sensor array system, designated Bio-LSI, to perform two-dimensional imaging of light-induced O₂ evolution from spinach leaves. The employed Bio-LSI chip consists of 400 sensor electrodes with a pitch of 250 μm for fast electrochemical imaging. Spinach leaves were illuminated to varying intensities of white light (400-700 nm) which induced oxygen evolution and subsequent electrochemical images were collected using the Bio-LSI chip. Bio-LSI images clearly showed the dose-dependent effects of the light-induced oxyge

SUBMITTER: Kasai S 

PROVIDER: S-EPMC6706413 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature